抗Flag tag抗体(anti-Flag tag antibody)

　　名称：抗Flag tag抗体(anti-Flag tag antibody)

　　货号：FNab03155

　　适应种属：重组蛋白

　　规格：100ug(微克)

　　状态：液体

　　纯化方法：蛋白A/G柱纯化

　　纯度：经sds-page分析，纯度大于95%

　　宿主：小鼠

　　亚型：IgG1

　　存储：用添加了0.02%叠氮化钠和50%甘油的pbs溶解，ph值7.3，可在-20℃下保存12个月（避免反复冻融）

　　免疫原：Flag tag

　　验证应用：ELISA, WB, IP

　　建议稀释比例：WB: 1:500-1:2000; IP: 1:500-1:2000

　　克隆性：单克隆

　　背景介绍：Protein tags are protein or peptide sequences located either on the C-or N-terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This antibody is generated against 1xDDDDK tag (DYKDDDDK) and can recognize protein containing one or more DDDDK tags, independently on N-terminal, C-terminal or internal regions of the target protein.

　　IP Result of anti-Flag tag (IP: FNab03155, 3ug; Detection: FNab03155 1:2000) with Recombinant protein protein lysate 500ug。

　　Flagged fusion protein were subjected to SDS PAGE followed by western blot with FNab03155(Flag tag antibody) at dilution of 1:5000。