抗ALPL抗体(anti-ALPL antibody)

　　名称：抗ALPL抗体(anti-ALPL antibody)

　　货号：FNab00347

　　适应种属：人源，大鼠，小鼠

　　规格：100ug(微克)

　　状态：液体

　　纯化方法：免疫亲和纯化

　　纯度：经sds-page分析，纯度大于95%

　　宿主：兔

　　亚型：igg

　　存储：用添加了0.02%叠氮化钠和50%甘油的pbs溶解，ph值7.3，可在-20℃下保存12个月（避免反复冻融）

　　免疫原：alkaline phosphatase, liver/bone/kidney

　　免疫原别称：Alkaline phosphatase, Alkaline phosphatase liver/bone/kidney isozyme, Alkaline phosphatase tissue nonspecific isozyme, ALPL, BLAP, Bone Alkaline Phosphatase, NBAP

　　分子量：60-80 kDa

　　验证应用：ELISA, WB, IHC, FC

　　建议稀释比例：WB: 1:500-1:3000; IHC: 1:20-1:200

　　克隆性：多克隆

　　uniprot id：P05186

　　背景介绍：There are at least four distinct but related alkaline phosphatases: intestinal, placental, placental-like, and liver/bone/kidney(tissue non-specific). The product of this gene is a membrane bound glycosylated enzyme that is not expressed in any particular tissue and is, therefore, referred to as the tissue-nonspecific form of the enzyme. ALPL(Alkaline phosphatase, tissue-nonspecific isozyme) is also named as AP-TNAP, TNSALP and belongs to the alkaline phosphatase family. It can exsit as a homodimer with the molecular weight of 140 kDa(PMID:18724009). ALPL was synthesized as a 66-kDa endo-b-N-acetylglucos-aminidase H(Endo H)-sensitive form, and processed to an 80-kDa mature form, which is anchored to the plasma membrane via glycosylphosphatidylinositol(GPI)(PMID:10839996).This antibody can bind the four mentioned alkaline phosphatases。

　　Immunohistochemistry of paraffin-embedded human colon cancer using FNab00347(ALPL antibody) at dilution of 1:50。

　　HEK-293 cells were subjected to SDS PAGE followed by western blot with FNab00347(ALPL antibody) at dilution of 1:800。