抗ZO-1抗体(anti-ZO-1 antibody)

　　名称：抗ZO-1抗体(anti-ZO-1 antibody)

　　货号：FNab09751

　　适应种属：人源，小鼠，大鼠

　　规格：100ug(微克)

　　状态：液体

　　纯化方法：免疫亲和纯化

　　纯度：经sds-page分析，纯度大于95%

　　宿主：兔

　　亚型：igg

　　存储：用添加了0.02%叠氮化钠和50%甘油的pbs溶解，ph值7.3，可在-20℃下保存12个月（避免反复冻融）

　　免疫原：tight junction protein 1(zona occludens 1)

　　免疫原别称：Tight junction protein 1, Tight junction protein ZO 1, TJP1, ZO 1, ZO1, ZO-1, Zona occludens protein 1, Zonula occludens protein 1

　　分子量：230kd

　　验证应用：ELISA, WB, IHC, FC, IF, IP

　　建议稀释比例：WB: 1:200-1:2000; IP: 1:200-1:2000; IHC: 1:50-1:200; IF: 1:20-1:200

　　克隆性：多克隆

　　uniprot id：Q07157

　　背景介绍：Tight junction(or zonula occludens) form the continuous intercellular barrier between epithelial and endothelial cells, which is required to separate tissue spaces and regulate selective movement of solutes across the epithelium and endothelium(PMID: 20066090). ZO-1(also known as TJP1) is a peripheral membrane phosphoprotein located on the cytoplasmic face and is expressed in tight junctions of both epithelial and endothelial cells(PMID: 3528172). It binds the transmembrane proteins occludin and the claudins linking them to cytoskeletal actin(PMID: 17418867). ZO-1 belongs to a family of multidomain proteins known as the membrane-associated guanylate kinase homologs(MAGUKs). It is a pivotal tight junction protein and may be involved in signalling mechanisms regulating cell proliferation and differentiation(PMID: 22782886)。

　　Immunohistochemistry of paraffin-embedded human kidney using FNab09751(ZO1 antibody) at dilution of 1:100。

　　Immunofluorescent analysis of MCF7 cells using FNab09751(ZO-1 Antibody) at dilution of 1:100。

　　IP result of anti-ZO-1(IP:FNab09751, 4ug; Detection:FNab09751 1:500) with HEK-293 cells lysate 2320 ug。

　　HepG2 cells were subjected to SDS PAGE followed by western blot with FNab09751(ZO1 antibody) at dilution of 1:500。