Anti-ILVBL antibody LM891143R

　　Anti-ILVBL antibody

　　产品描述

　　Anti-ILVBL antibody

　　分子量68 KDa

　　种属反应性Human,Mouse,Rat,Dog,Pig,Horse

　　验证应用WB,FC

　　抗ti类型兔多抗

　　免疫原KLH conjugated synthetic peptide derived from human ILVBL 301-400/632

　　偶联Non-conjugated

　　性能

　　形态Liquid

　　浓度1 mg/mL.

　　存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

　　存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

　　亚型IgG

　　纯化方式Protein A affinity purified.

　　亚细胞定位Membrane.

　　数据链接SwissProt: A1L0T0 Human

　　其它名称

　　more

　　Acetolactate synthase homolog

　　Acetolactate synthase like protein

　　Acetolactate synthase-like protein

　　应用

　　WB:1:500-2000

　　FC:3ug/Test

　　Fig1: Sample: HepG2 Cell (Human) Lysate at 30 ug

　　Primary: Anti-ILVBL at 1/300 dilution

　　Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

　　Predicted band size: 68kD

　　Observed band size: 68kD

　　Fig2: Blank control: HepG2.

　　Primary Antibody (green line): Rabbit Anti-ILVBL antibody (

　　Dilution: 1μg /10^6 cells;

　　Isotype Control Antibody (orange line): Rabbit IgG .

　　Secondary Antibody : Goat anti-rabbit IgG-PE

　　Dilution: 1μg /test.

　　Protocol

　　The cells then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

　　Fig3: Sample:

　　Hcclm3(Human) Cell Lysate at 30 ug

　　MCF-7(Human) Cell Lysate at 30 ug

　　A431(Human) Cell Lysate at 30 ug

　　Primary: Anti- ILVBLt 1/1000 dilution

　　Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution

　　Predicted band size: 68 kD

　　Observed band size: 68 kD

　　本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！