Anti-Calponin antibody SI67-01 LM860617R

　　Calponin regulates smooth muscle cell contraction and is a marker of smooth muscle cell differentiation. Calponin, an Actin- and Tropomyosin-binding protein, is characterized as an inhibitory factor of smooth-muscle actomyosin activity. Calponin is implicated in the regulation of smooth muscle contraction through its interaction with F-Actin and inhibition of the Actin-activated MgATPase activity of phosphorylated Myosin. Both properties are lost following phosphorylation (primarily at Serine 175) by protein kinase C or calmodulin-dependent protein kinase II. The three forms of Calponin, Calponin 1 (basic Calponin), Calponin 2 (neutral Calponin) and Calponin 3 (acidic Calponin), are found in smooth muscle tissue. Additionally, Calponin 2 is found in heart muscle tissue and Calponin 3 is found in the brain.

　　产品名称Anti-Calponin antibody [SI67-01]

　　分子量33 kDa

　　种属反应性Human, Mouse, Rat, Dog, Pig

　　验证应用WB, ICC/IF, IHC-P

　　抗ti类型重组兔单抗

　　免疫原Synthetic peptide within C-terminal human Calponin.

　　偶联Non-conjugated

　　性能

　　形态Liquid

　　浓度1 mg/mL.

　　存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

　　存储缓冲液1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

　　亚型IgG

　　纯化方式Protein A affinity purified.

　　亚细胞定位Cytoskeleton.

　　数据链接SwissProt: P51911 Human

　　SwissProt: Q08091 Mouse

　　SwissProt: Q08290 Rat

　　SwissProt: Q08092 Pig

　　其它名称

　　Basic calponin antibody

　　Calponin 1 antibody

　　Calponin 1 basic smooth muscle antibody

　　应用

　　WB: 1:500-1:2,000

　　ICC/IF: 1:50-1:200

　　IHC-P: 1:50-1:200

　　Fig1: Western blot analysis of Calponin on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

　　Positive control:

　　Lane 1: Hela cell lysate

　　Lane 2: NIH/3T3 cell lysate

　　Fig2: ICC staining of Calponin in HepG2 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the primary antibody for 1 hour at room temperature, washed with PBS. Alexa Fluor?488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/1,000 dilution. The nuclear counter stain is DAPI (blue).

　　Fig3: Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Calponin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

　　Fig4: Immunohistochemical analysis of paraffin-embedded human bladder tissue using anti-Calponin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody ( for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

　　Fig5: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Calponin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

　　Fig6: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Calponin antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

　　本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！