Anti-TRIM25 antibody LM800104R

anti-trim25 antibody

产品描述

　　the protein encoded by this gene is a member of the tripartite motif (trim) family. the trim motif includes three zinc-binding domains, a ring, a b-box type 1 and a b-box type 2, and a coiled-coil region. the protein localizes to the cytoplasm. the presence of potential dna-binding and dimerization-transactivation domains suggests that this protein may act as a tranion factor, similar to several other members of the trim family. expression of the gene is upregulated in response to estrogen, and it is thought to mediate estrogen actions in breast cancer as a primary response gene.

　　产品名称anti-trim25 antibody

　　分子量predicted band size: 71 kda.

　　种属反应性human,rat

　　验证应用wb,ihc-p

　　抗ti类型兔多抗

　　免疫原recombinant protein within human trim25 aa

　　偶联non-conjugated

性能

　　形态liquid

　　浓度1 mg/ml.

　　存放说明store at +4℃ after thawing. aliquot store at -20℃. avoid repeated freeze / thaw cycles.

　　存储缓冲液1*tbs (ph7.4), 0.2% bsa, 50% glycerol. preservative: 0.05% sodium azide.

　　亚型igg

　　纯化方式protein affinity purified.

　　亚细胞定位cytoplasm, stress granule.

　　数据链接swissprot: q14258 human

　　其它名称

　　e3 ubiquitin/isg15 ligase trim25 antibody

　　efp antibody

　　estrogen responsive finger protein antibody

　　more

应用

　　wb: 1:500-1:2,000

　　ihc-p: 1:100-1:500

　　fig1: western blot analysis of trim25 on different lysates. proteins were transferred to a pvdf membrane and blocked with 5% bsa in pbs for 1 hour at room temperature. the primary antibody was used in 5% bsa at room temperature for 2 hours. goat anti-rabbit igg - hrp secondary antibody (ha1001) at 1:5,000 dilution was used for 1 hour at room temperature.

　　positive control:

　　lane 1: jurkat cell lysate

　　lane 2: 293 cell lysate

　　fig2: western blot analysis of trim25 on sk-br-3 cell lysates. proteins were transferred to a pvdf membrane and blocked with 5% bsa in pbs for 1 hour at room temperature. the primary antibody was used in 5% bsa at room temperature for 2 hours. goat anti-rabbit igg - hrp secondary antibody (ha1001) at 1:5,000 dilution was used for 1 hour at room temperature.

　　fig3: immunohistochemical analysis of paraffin-embedded rat bladder tissue using anti-trim25 antibody. the section was pre-treated using heat mediated antigen retrieval with tris-edta buffer (ph 8.0-8.4) for 20 minutes.the tissues were blocked in 5% bsa for 30 minutes at room temperature, washed with ddh2o and pbs, and then probed with the primary antibody for 30 minutes at room temperature. the detection was performed using an hrp conjugated compact polymer system. dab was used as the chromogen. tissues were counterstained with hematoxylin and mounted with dpx.

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！