Anti-GDF1 antibody LM800058R

Anti-GDF1 antibody

产品描述

　　Growth differentiation factor 1 (GDF1) is a protein that in humans is encoded by the GDF1 gene. This gene encodes a member of the bone morphogenetic protein (BMP) family and the TGF-beta superfamily. This group of proteins is characterized by a polybasic proteolytic processing site that is cleaved to produce a mature protein containing seven conserved cysteine residues. The members of this family are regulators of cell growth and differentiation in both embryonic and adult tissues. Studies in rodents suggest that this protein is involved in the establishment of left-right asymmetry in early embryogenesis and in neural development in later embryogenesis. This protein is transcribed from a bicistronic mRNA that also encodes the longevity assurance gene.

　　产品名称Anti-GDF1 antibody

　　分子量Predicted band size: 39 kDa.

　　种属反应性Human,Rat

　　验证应用WB,IHC-P

　　抗ti类型兔多抗

　　免疫原Synthetic peptide within C-terminal human GDF1.

　　偶联Non-conjugated

性能

　　形态Liquid

　　浓度1 mg/mL.

　　存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

　　存储缓冲液1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

　　亚型IgG

　　纯化方式Peptide affinity purified.

　　亚细胞定位Secreted.

　　数据链接SwissProt: P27539 Human

　　其它名称

　　DORV antibody

　　DTGA3 antibody

　　Embryonic growth/differentiation factor 1 antibody

　　more

应用

　　WB: 1:500-1:1,000

　　IHC-P: 1:100-1:500

　　Fig1: Western blot analysis of GDF1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

　　Positive control:

　　Lane 1: SH-SY5Y cell lysate

　　Lane 2: MCF-7 cell lysate

　　Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-GDF1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody for 30 minutes at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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