Rabbit anti-Rat IgG(H+L)-HRP antibody LM8022R

　　Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.

　　产品名称Rabbit anti-Rat IgG(H+L)-HRP antibody

　　种属反应性Rat

　　验证应用WB, ELISA

　　抗ti类型兔多抗

　　免疫原Rat IgG(H+L)

　　偶联HRP-conjugated

性能

　　形态Liquid

　　浓度2 mg/ml.

　　存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

　　存储缓冲液1*TBS (pH7.4), 0.5%BSA, 40% Glycerol.

　　亚型IgG

　　纯化方式Protein affinity purified.

应用

　　WB: 1:5,000-1:10,000

　　ELISA: 1:10,000

本产品只适用于科研，不能用于临床诊断。严禁用于临床医疗及其他非科研用途！